2026-09-18T19:03:58Zhttps://api.mountainscholar.org/server/oai/request

oai:mountainscholar.org:10217/2383602026-06-11T19:48:23Zcom_10217_100532com_10217_100000com_10217_100357com_10217_100303col_10217_182111col_10217_100359

Roessler, Ryan, author Krapf, Diego, advisor Stasevich, Tim, committee member Prasad, Ashok, committee member 2024-05-27T10:31:47Z 2026-05-20 2024 Roessler_colostate_0053N_18188.pdf https://hdl.handle.net/10217/238360 https://doi.org/10.25675/3.02348 Information about the diffusive motion of RNA would provide insights into intracellular structures and functions, as well as gene expression and genetic regulation. We study the motion of individual messenger RNA molecules in the cytoplasm of HeLa cells. RNAs are imaged in live cells via total internal reflection (TIRF) microscopy. In order to visualize individual RNA molecules expressing the MYH9 gene, they were labeled via MS2 stem loops bound to coat proteins tagged with the HaloTag-JF646 fluorophore. We then used single-particle tracking to obtain trajectories of individual molecules. Trajectories were analyzed in terms of their mean-squared displacement (MSD) and power spectral density (PSD). We observed non-ergodic, subdiffusive behavior, with statistics that depend on observation time, i.e., aging. Additionally, we observe stochastic switching between two mobility states with an order of magnitude difference in diffusivity. This switching process is responsible for the aging nature of the system. When compared to the cytoplasmic motion of synthetic nanoparticles, the analysis of RNA trajectories gives rise to discrepancies that raise questions about specific intracellular interactions. born digital masters theses English eng Colorado State University. Libraries 2020- Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. Anomalous diffusion of mRNA in the cytoplasm of HeLa cells Text 2026-05-20 2026-05-20 This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). Master of Science (M.S.) Masters Biomedical Engineering Colorado State University open.access